The aim of present work was to prepare hand sanitizer using natural antimicrobial agent and moisturizer. A clean and hygienic manufacturing environment is an essential prerequisite in order to keep contamination related reject rates low. In optimized base formulation, natural antimicrobial agents and moisturizing agent in different concentration was incorporated and subjected to various evaluation tests. Antibacterial activity of alcohol:water (65:35) and sanitizers was carried out by disk diffusion method against two human pathogenic bacteria, including Gram positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia Coli). The moisturizing activity was determined by consideration of moisture content, which was carried out with the help of Karl Fischer apparatus. Formulation containing 0.5% of carbopol was found with high viscosity hence continued the experiment by optimizing one base formulation amongst others. Zone of inhibition showed antibacterial activity with different zone of inhibition measured in mm and observed that neem extract has good antibacterial activity than ginger extract. The moisturizing activity studied by moisture content test, indicated that all the formulations were shown good moisture content with sufficient moisture content. Formulation AF2 and AF3 having good consistency and spreadability with pH in between 5.5 to 6.5. An optimized formulation AF2 and AF3 show good antibacterial activity. Hence antibacterial activity from natural sources is best alternative for the synthetic one.
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